evaluation of association between HLA and disease progression among HIV/ADIS patients referred to Imam Khomeyni Hospital

ABSTRACT

Background: Human leukocyte antigen [HLA] class I and class II is believed to play a major role in the immune response to HIV, and are known to be associated with rates of progression to AIDS. This study measured the rates of variation within well-defined epitopes to determine associations with the HLA-linked exposure of disease progression. The aim of this study was to evaluate the association between HLA and disease progression among Iranian HIV/AIDS patients in comparison with general population. Materials and methods: In this case- control study, the role of HLA class I [A, B] and class II [DRB1, DQA1 and DQB1] alleles were investigated in 48 HIV infected Iranian patients compared with 100 healthy individuals. HLA typing was carried out by PCR amplification using sequence specific primers [PCR-SSP]. The primer was supplied by the standard kit. The disease progression was determined based on changes in stage of disease. Results: Among 48 HIV infected patients and the control group a>positive association was observed with HLA- DRB1* 0301 [1.32< OR< 7.09, P= 0.0037], HLA- DQA1 * 0501[1.01< OR< 4.97, P=0.03], HLA- DQB1 * 0201 [0.98< OR< 3.50 P= 0.04] as well as a negative association with HLA- DRB1 * 1301 [0.01< OR< 0.98, P= 0.022] in HIV progression. In this study, no significant association was found with the analyzed HLA-B*2701 and HLA-B* 5701 alleles in the case of HIV progression. Conclusion: In our study among HIV infected patients the HLA- DRB1 * 0301, HLA- DQA1 * 0501 and HLA- DQB1 * 0201 were the most important predictors for the HIV progression, whereas, HLA- DRB1 *1301 detected as strong protectors. As the results of the limitations in present study such as being small sample size, studies among a larger population would be necessary to in HIV infected patients with predisposing HLA alleles for early therapy