Molecular epidemiology and phylogenetic analysis of Dengue virus type-1 and 2 isolated in Malaysia

ABSTRACT

Objective: Detection of different serotypes of dengue virus and provide information on origin, distribution and genotype of the virus. Methods: Dengue virus serotypes identified as DEN-1 and DEN-2 were amplified and sequenced with E gene. The consensus sequences were aligned with references E gene sequences of globally available GenBank. Phylogenetic analysis was performed using Neighbor-joining and Kimura 2-parameter model to construct phylogenetic tree. Results: A total of 53 dengue virus isolates were positive, of which 38 [71.7%] were DENV-1 and 15 [28.3%] were DENV-2. Phylogenetic tree of DENV-1 and DENV-2 showed that the isolates were clustered in genotype I and cosmopolitan genotype, respectively considered the predominant genotypes in Southeast Asian countries. The molecular epidemiology genotype I DENV-1 and cosmopolitan genotype DENV-2 have been co-circulating in Klang Valley areas, Malaysia without shifting of genotype. Conclusion: The study reveals that DENV-1 and DENV-2 have been circulating in Malaysia. The isolates are clustered in genotype 1 and cosmopolitian genotype, respectively. The study results would help in planning for prevention and control of dengue virus in Malaysia