Oxytocin can decrease germ cells dapsgtotic index in testis under acute ischemia reperfaasion in a rat model

ABSTRACT

Background: Ischemia reperhs on [IR] is the main pathology of torsion of testis I and it is a common urologic emergency. There is some evidence that shows oxytocin I [OT] plays role in ischemia reperhsion. Objective: To evaluate this hypothesis that OT can decrease germ cell apoptotic index in testis under acute ischemia reperfusion in arat model. Materials and Methods: 20 adult rats were randomly divided into four groups I Control, IR, OT and IF.+ OT [OTA]. Testicular ischemia was achieved by 720" torsion of the left testis for 2 hr. Then, torsion was removed and reperfusion was performed. Immediately after induction of reperfusion 0.03 pgkg OT were administered intraperitoneally to the IR+ OT. Three hours after surgery left testis I was removed and evaluations were made by Johnson's score, ELISA, immunohistochemistry and histomorphometry for study of maturity of spermatogenesis, endocrine profiles, apoptosis and quantitative studies, respectively. Results: The results showed in addition tissue edema and congestion, a significant reduced in Johnson's score were detected in IR group in comparison with controls [p=0.01], and apoptotic index increased significantly @=0.001]. Administration of OT in OT+IR group, increased Johnson's score but it was not statistically significant. Germinal epithelium thickness was increased significantly [p=0.03], although apoptotic index decreased significantly in comparison with the IR group [p=0.04]. However there was not significant difference in serum levels of I testosterone, FSH and LH innone of groups [p=0.07].Conclusion: These results suggested that OT can decrease apoptotic index and improves complication of a cute ischemic reperftlsion in testis in a rat model