reversed phase high performance liquid chromatographic method for determination of rapamycin

ABSTRACT

Easily degradating and various isomeric forms of rapamycin [Sirolimus] face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic [RP-HPLC] method for rapamycin. Separation was performed on a C[8] column [MZ, 15 × 4.6 mm, 5 [micro]m particle size] using methanolwater [8020 v/v] as the mobile phase with the flow rate of 1 mL/min. The column temperature was set at 57degreeC and the detection was carried out at the wavelength of 277 nm. The method was linear over a concentration range of 0.025-2 [micro]g/mL. The coefficient of variation of intra- and inter-day, assessed at three concentration levels of 0.075, 0.3 and 0.900 [micro]g/mL, was less than 2%. Limit of quantification [LOQ] was found 25 ng/mL. The method with high percent recovery and short retention time of rapamycin, was found to be simple, rapid and reproducible