role of some natural antioxidants in reducing the cytotoxic of cholesterol oxidized products [COPs] in rats exposed to H[2]O[2]

ABSTRACT

This study evaluated the effect of different types of antioxidants [selenium as sodium selenite, lyophilized green tea extract, lyophilized anthocyanins pigment of red grape mare extract, and lyophilized beta carotene pigment of carrot pulp waste extract] as antioxidants on the cytotoxic actions of oxysterols in plasma, brain and liver formed endogenously in rats exposed to H[2]O[2] [1 %], Rats were fed normal diet and exposed to H[2]O[2]-induced oxidative stress for 9 weeks. The concentrations of cholesterol oxidation products [COP] [25-hydroxycholesterol, 7 beta hydroxycholesterol and 7- ketocholesterol], were significantly increased in plasma and organs [brain and liver] in positive group, and significantly reduced by antioxidant treatment. Plasma· Malondialdehyde [MDA] and lactic acid dehydrogenase activity [LDH] were significantly increased, while the activity of superoxide dismutase [SOD] and catalase [CAT] activity were decreased in rats exposed to H[2]O[2] [1%]. The administrated antioxidants improved these values to the normal levels or near