Analgesic and anti-inflammatory activities of fixed oil of Macrotyloma uniflorum [Lam.) Verdc. in mice and rats

ABSTRACT

Macrotyloma uniflorum commonly known as horse gram or kulthi bean is grown as a pulse for livestock and human consumption. The beans contain about 1.3% fat, 18% protein, 15% carbohydrate along with vitamins and minerals. In traditional medicine it is used as antihyperglycemic, antioxidant, antihypertensive and diuretic. Other important medicinal uses include treatment of renal stones, obesity, piles, oedema and fever. The present study evaluated analgesic [by acetic acid induced writhing, hot plate and tail flick tests in mice] and anti-inflammatory [carrageenan induced paw edema in rats] activities of Macrotyloma uniflorum fixed oil [MUFO]. Four groups were included in study Group-I Normal Saline Control [2ml/kg], Group-II MUFO [2ml/kg], Group-III MUFO [4ml/kg], and Group-IV Standard Acetyl salicylic acid [ASA 300mg/kg]. All results were significant however delayed onset of action was observed in tail flick and paw edema tests. Acute oral toxicity of the oil was also checked in mice and was found safe up to 4ml/kg dose, as no signs of toxicity and mortality were observed. It is concluded that Macrotyloma uniflorum fixed oil may possess analgesic and anti-inflammatory activity which can be related with a peripheral mechanism of action