Study of pentoxifylline drug effect on Bax gene expression changes in kidney after ischemic/reperfusion in rat

ABSTRACT

Ischemia Reperfusion injury is the tissue damage caused when blood supply returns to the tissue after a period of ischemia or lack of oxygen. Ischemia Reperfusion induces cell death and endemic reaction that is one of the most important clinical problems with acute renal failure and renal transplantation. In this study, the effect of pentoxifylline on rat kidney function and cell injury following Ischemia Reperfusion were evaluated. In this experimental study, 20 male wistar rats with average weight of 250-300g were selected and then were accidently divided them on two tenth group of control and treatment groups. In the control group, celiotomy was performed by ventral midline incision. The left kidney was isolated, and then both the renal artery and vein were obstructed. After 60 minutes of warm ischemia, vessel obstruction resolved and the right kidney was removed. 72 hours after reperfusion, tissue samples were taken from left kidney for histopathology. All these steps in treatment group were exactly repeated after administration of 45 mg/kg/PO pentoxifylline [3 hours before operation] and in this group treatment was continued every 12h until 3 days. In this research quantitative real-time PCR is used for the detection expression Bax gene in ischemia group and PNT drug group and compared to normal sample .The results showed the gene dosage ratio of 1.24 for ischemia groups and 0.64 for drug group. The results showed the expression Bax gene in PNT group decline than to ischemia group. Therefore, quantitative real time PCR could be used as a direct method for detection of Bax gene expression in tested and normal samples