Analysis of interleukin-18 and CD14 gene polymorphism in atopic patients

ABSTRACT

Immunoglobulin E [IgE] mediated allergy is a common chronic disorder resulting from interaction between genetic and environmental factors. We aimed to reveal the single nucleotide polymorphisms' [SNPs] in 1L-18 [137G>C] and CD 14 [C-159T] genes to reveal their possible association with atopic diseases in Egyptian population. A total of 30 diagnosed atopic patients and a total of 30 age-matched control subjects were included in this study. Diagnosis of atopy was made according to the family history and the results of both intradermal skin test and total IgE enzyme- linked immunosorbent assay. Interleukin-18 [-137G>C] genotyping was performed by polymerase chain reaction with sequence specific primers [PCR-SSP] The CD14 [C-159T] polymorphism was detected by polymerase chain reaction -Restriction fragment length polymorphism [PCR-RFLP], Insignificant higher prevalence of heterozygous genotype [GC] of IL-18 [-137G>C] gene was found in atopic patients compared to control subjects. Statistical analysis revealed significant association between atopy and the CC genotype of CD14 [C-159T]. Only the genotype combination GG/ CC which is composed of the IL-18 GG genotype and CD 14 CC genotype was preferentially transmitted to atopic patients. The results of this study suggested a possible association of atopy with the CD 14 [C-159T]. Moreover, the genotype combination GG/ CC may be one of the factors that participate in the. pathogenesis of atopy?