[Cloning, bioinformatics study and evaluation expression of gene of enterotoxigenic Escherichia coli CFA/I major subunit [CfaB]]

ABSTRACT

Background and aims: Enterotoxigenic Escherichia coli [ETEC] is the main cause of diarrhea in children in developing countries and also in travelers to these areas. ETEC attaches to host cells via filamentous bacterial surface structures, known as colonization factors. Epidemiological studies suggest that the prevalence of CFA/I is higher than other colonization factors. CFA expressed by ETEC and so represents an important component of any vaccine. Investigation supposed that CfaB as a major subunit of fimbriae is an appropriate candidate for vaccine preparation. In the present study we investigated cloning and expression of CfaB