Hepatoprotective effect of C-phycocyanin against carcinogenic Di-methylnitrosamine substance

ABSTRACT

Hepatotoxicicity of dimethylnitrosamine [DMNA] and counteraction effects by phycocyanin were evaluated in an experiment using male rats [100-120g] for each. Six groups 10 animals for each were selected. All animals were intraperitonally injected. The first one injected with saline phosphate buffer serve as control. The second injected with phycocyanin at the dose [300 mg/kg body weight]. The third with dimethylnitrosamine at the dose [10mg/kg body weight]. The fourth injected with C-PC then one week later injected with DMNA, where the fifth injected with DMNA THEN one week later injected with CPC. The last one injected with a mixture of C-PC and DMNA. All the animals were housed for 30 days then, sacrificed .Blood and plasma were collected and prepared for biochemical analysis. Liver was excised and subjected to histopathological examination. RESULTS showed that cpc inhibited the increases in plasma ALT; AST by DMNA.AMNA also caused obvious decreases in both protein and albumin to the minimum levels. Moreover DMNA induced a marked reductions in the antioxidant enzymes activities in serums of rats especially superoxide dismutase and catalase enzymes. The percentages of their activities were less than their controls by 31%and 48%respectivelly. The previous activities were elevated to normal levels as the animal exposed to injection with C-PC. Regarding to the production of immunoglobulin, it could be obvious that DMNA suppress IgM and IgG to the minimum levels [62 and 52%] less than their irrespective controls. IgA was also decreased to the level of 32%. C-PC injection after DMNA has antagonistic effects to the serious response of DMNA. It enhanced the production of immunoglobulin more than control animals with special references to IgM. Histopathological examination confirmed that DMNA has hepatotoxicity since, the hepatocytes showed vascular and nuclear degeneration with different sign of congested blood sinusoids andVon Kupffer cells. The pathological responses of DMNA were dimensioned with the administration of C-PC where hepatocytes appeared normal with normal vesicular nuclei. The cells had well distinct endothelium of the central vein, normal cytoplasm constituents with no congestion in the sinusoid