[Studies on transformation of resistant plasmid between salmonella and e. col1]

ABSTRACT

Plasmid profile analysis of 10 multiple drug resistant Salmonella serovars with [resistant to am-picillin, chloramphenicol, oxytetracycline, nali-dixic acid and streptomycin] was performed using agarose gel electrophoresis. There were 8 isolates harbouring plasmid with molecular weight ranged from 16 - 31.5 kbp while the plasmid of 2 isolates could not be detected. Successful transformation of resistant plasmid was done between S. Kentucky and S. Typhirnurium as donor bacteria and E. coli XL1 as recipient bacteria by electroporation. Transconjugants were obtained by conjugation assay between multiple drug resistant [MDR] S. Typhimurium as donor bacteria and each of S. Enteritidis , S. Typhi, E. coli XL1 and E. coli O157H7 as recipient bacteria?