Medium composition influencing single cell protein and cholesterol production by saccharomyces cerevisiae var carlsbergensis

ABSTRACT

Sugarcane molasses was found to be good carbon source for growth and production of single cell protein [SCP] by Saccharomyces cerevisiae var. carlsbergensis. Appreciable SCP yield was attained in the presence of molasses at 1 percent sugar concentration, whereas 5 percent was coupled with a good growth and minimal cholesterol content. The addition of 0.5 percent urea as a nitrogen source allowed better fermentation yield and suppressed cholesterol production. Further improvement of biomass and SCP production was achieved on adding 0.3 percent KH[2] PO[4] and 0.15 percent Mg SO[4]. 7H[2]O to the molasses medium. The comparative analysis of yeast cells grown on the synthetic [Phaff] medium with that grown on optimized molasses medium revealed a percentage increase in the later case of 415 percent SCP content, 527 percent peptides, 1018 percent amino acids, 201 percent ammonia, 394 percent total soluble nitrogen, 394 percent total nitrogen and 522 percent in dry weight gain. The cholesterol content decreased by 40 percent on molasses medium