Some factors affecting biosynthesis of fibrinase by oidiodendron flavum

ABSTRACT

It was shown that the activity of fibrinolytic exoprotease in culture of the thermophilic fungus Oidiodendron flavum depended on the nutritional composition of the medium. A potent extracellular fibrinolytic enzyme was obtained when fibrin [2/g/l] used as a carbon source, sugar addition decreased the enzyme production. Further increase in fibrinase yield was recorded when 0.05 g/1 NH[4]NO[3] was added to the medium. Among the tested phosphate sources, KH[2]PO[4] at 1.0 g/1 was optimum for enzyme production. [NH[4][2]]SO[4] at 0.5 g/1 led to significant activation of fibrinolysis either in growth medium or in reaction mixture