Histological and histochemical evaluation of the reaction of demineralized-freeze dried bone and med for porous polyethylene implant materials to bone during healing of osseous defects

ABSTRACT

The present study was performed to evaluate the histological reaction of two graft materials both are widely used clinically Demineralized freeze-drired bone [DFDBA] and Medpor on bone. They are different in chemical and physical composition. 15 rabbits [males] were used in this study. Osseous defects were created in the buccal side of their mandibles. Then the animals were divided into 3groups group I [control], group II [study] that included osseous defects filled with DFDBA, group III [study] which included osseous defects filled with Medpor. The experimental animals were sacrificed in intervals 2,6,12 weeks. Histological and histochemical results revealed successful bone healing in all groups. DFDBA group showed a number of inflammatory cell at the area of osseous defect after 2 weeks. Followed by formation of woven bone between the bone graft material then progressive bone formation, maturation and regeneration were observed. The mature bone was greater in amount than the two orther groups, fused completely to the host bone. The DFDBA was completely resorbed by the end of the experiment. The Med por group showed few inflammatory cells in 2 weeks then new bone formation and fibrous tissue in growth between its pores were observed together with areas of fusion to the host bone. By the end of the follow up period dense mature bone trabeculae attached to the Med por particles were forming a dense mass fused completely to the host bone. The control group showed normal bone healing less in amount than the DFDB group and less indense than the Med por group. Alkaline phosphatase enzyme activity was increased with the period of follow up marked on the surface of the host bone facing the graft materials and on the bony trabeculae between the materials particles of the both DFDB and Med por