[ inhibitory effects of garlic and pan ax ginseng extracts against the genotoxicity, biochemical and histological changes induced by ethylene- diaminetetraacetic acid [EDTA] in male rats]

ABSTRACT

EDTA is widely used in food and other industries to sequester metal ions and to prevent their disadvantageous effects. The objective of the current study was to evaluate the protective effect of Panax ginseng and garlic against EDTA-induced biochemical, cytogenetic and histological changes in rats. Forty male rats were divided into eight treatment groups. They were treated for 7 days as follow the control group, the group treated with EDTA [20 mg/kg b.w] and the groups treated with Panax ginseng extract [20 mg/kg b.w], garlic [5 mg/kg b.w], panax ginseng plus garlic alone or in combination with EDTA. In vivo bone marrow micronucleus test and random amplified polymorphism DNA [RAPD] method were performed to assess the antigenotoxic effect of both protective agents. The results indicated that EDTA administration caused a significant decrease in the serum biochemical parameters, Alanine aminotransferase [ALT], Aspartate aminotransferase [AST], Lactate dehydrogenase [LDH] Blood urea nitrogen [BUN]. Increased the incidence of Micronucleated Polychromatic Erythrocytes [MnPCEs] as well as appearance of some changes in polymorphism band patterns, and induced different histopathological lesions in the livers, kidneys and testis Treatment with Panax ginseng, garlic alone or plus EDTA significantly improved all the tested parameters. Moreover, Panax ginseng extract was found to be more effective than garlic in restoring the parameters that were altered by EDTA