Biochemical functions and pharmaceutical and clinical analysis of nicotinamide

ABSTRACT

The major biochemical role of nicotinamide [vitamin 63] is its involvement in redox reactions and energy metabolism. The biological forms of nicotinamide are nicotinamide adenine dinucleotide [NAD] and nicotinamide adenine dinucleotide phosphate [NADP] coenzymes. Nicotinamide is a component of vitamin B-complex and is used in combination with other vitamin B compounds in pharmaceutical preparations. Several analytical methods have been used for the assay of nicotinamide in commercial products. These methods include high-performance liquid cinematography [HPLC], mass spectrometry in the multiple reaction modes [LC/UV/MS/MRM], liquid chromatographic-isotope detection mass spectrometry [LC/DMS], and planner chromatography-multiple detection by electro-spray mass spectrometry [ES/MS], capillary zone electrophoresis [CZE], thin-layer chromatography [TLC]-densitometry, differential pulse polarography and cyclic voltametry. The clinical analysis of nicotinamide involves the application of liquid chromatography-tandem mass spectrometry [LC-MS/MS], high-performance liquid chromatography [HPLC] and capillary electrochromatgraphy