Stability indicating method for the quantitation of trazodone hydrochloride in tablet form

ABSTRACT

A thin layer chromatographic separation followed by dye-salt partition technique was employed to estimate the antidepressant drug trazodone hydrochloride in its tablet preparations. The chromatographic separation was carried out on silica gel plates using cyclohexane- benzene-diethylamine [5 4 1] as a mobile phase. The separated drug is determined colorimetrically using two acid dyes [tropeolin OO and bromocresol green]. Chloroform was used as a solvent for the extraction of the formed ion pair. Absorbances were measured at 540 nm and 620 nm after libration of the free dye from the formed dye- salt. Beer's law was obeyed over the concentration ranges of 2-8 ug ml-1 and 8-24 ug ml-1, respectively