Ethanolic seeds extract of Centratherum anthelminticum reduces oxidative stress in type 2 diabetes

ABSTRACT

This work was accomplished to assess the in-vitro antiglycation and antioxidant activities of ethanolic seeds extract of Centratherum anthelminticum [CSEt], followed by its in-vivo examination in type 2 diabetes. Overnight fasted rabbits were divided into control and diabetic groups. Rabbits in diabetic group were fed with 35% fructose solution to develop hyperglycemia that was well-monitored by glucometer. These were divided into diabetic control [distilled water 1ml/kg], positive control group [pioglitazone 15mg/kg] and two test groups [CSEt 400 and 600mg treated]. All treatments were given orally. After 14 days, rabbits were sacrificed and blood samples were used to estimate glycated haemoglobin [HbA1c] while total bilirubin [direct and indirect], uric acid, alanine aminotransferase [ALT] and creatine kinase [CK] were done in sera. In addition, antioxidant parameters viz., catalase [CAT], superoxide dismutase [SOD], reduced glutathione [GSH] and lipid peroxidation [LPO] were in liver tissues. The in-vitro studies showed good antiglycation and antioxidant potential of CSEt. Similarly, in-vivo investigation showed significant reduction in glycemia and body weights in type 2 diabetic test groups. Plus values of HbA1c, ALT, CK, uric acid and bilirubin were almost back to normal along with improvement found in efficiency of antioxidant parameters