Drug-induced hepatotoxicity: study the effect of sulphonylurea on the labilization of four marker lysosomal enzymes in rat liver in-vitro

ABSTRACT

We have studied the effect of tolbutamide as a first generation sulfonylurea, and gliclazide as a second generation sulfonylurea on the labilization of four market rat liver lysosomal enzymes namely Acid phosphatase [ACP], beta-Galactosidase [beta-GAL], N-Acetyl beta Glucosaminidase beta-NAG] and beta-Glucuronidase [beta-GLU]. Three therapeutic concentrations of these drugs were used after three incubation periods 30, 60 and 120 minutes The results indicated that both tolbutamide and gliclazide exhibited a marked increase in the labilization of the four lysosomal enzymes. The labilizing effect of gliclazide was greater than that of mlhutamide especially on ACP, beta-NAG, while, beta-GLU activity was changed. The labilizing effect of these drugs on the lysosomal membrane was time and dose dependent