Simultaneous analysis of trimethoprim and sulphamethoxazole drug combinations in dosage forms by high performance liquid chromatography

ABSTRACT

A sensitive high performance liquid chromathography [HPLC] analytical procedure was developed for the quantitive determination of trimethoprim [TM] and sulphamethoxazole [SM] in commercial dosage forms. C18 analytical column [stainless steel, 25 cm X 4.6 mm i.d.] was packed with 5-micro m particles of the reversed phase material and used for assays. Mobile phase containing 0.025 M sodium phosphate as aqueous phase and acetonitrile with 0.4% triethylamine as organic phase. The drugs were quantified at flow-rate of 1.2 ml/min, with ultraviolet detection at 260 nm. The minimum detectable quantities in assays were 100 ng/ml for SM and 75 ng/ml for TM. The method is well suited to routine application and adequate sensitivity with precision