[Evaluation of two commercial qualitative and semi quantitative ELISA methods for diagnosis of acute human leptospirosis]

ABSTRACT

Introduction: Leptospirosis is most widespread Zoonosis in the world, especially in Tropical and temperate regions with moist climate. Diagnosis of Leptospirosis according to clinical symptoms is difficult and uncertain due to lack of specific sign [s]. Leptospira is a fastidious bacterium. Isolation of these bacteria by culture is difficult, time consuming and hence doses not contribute to an early diagnosis. Specific antibodies against Leptospira appear from 6th day of disease onset. The Microscopic Agglutination Test [MAT] is most reliable assay but generally requires paired sera for detection of seroconversion and is considered too complex for routine use