Enhancement of tropane alkaloids production in Egyptian henbane [Hyoscyamus muticus L.] via Agrobacterium rhizogenes transformation

ABSTRACT

An efficient protocol for establishment of transgenic Egyptian henbane [Hyoscyamus muticus L.] root cultures using Agrobacterium rhizogenes is reported. Two strains of A. rhizogenes 15834 and A4, carrying the pBI121 binary vector, we're tested for their ability to produce hairy roots on wounded hypocotyl, leaf and stem explants as well as complete seedlings. One of the strains induced hairy root formation on all explants, whereas the other type caused the growth of tumorigenic calli or produced no response. The effectiveness of different concentrations of Kanamycin as antibiotic for selective medium of hairy root production was investigated. On other hand, the effect of transgenic hairy root cultures an enhancement of total tropane alkaloids, hyoscine and hyoscyamine production was investigated as compared with the production of tropane alkaloids from different calli cultures obtained