Soluble expression of humanized anti-cd20 single chain antibody in escherichia coli by cytoplasmic chaperones co-expression

ABSTRACT

Background: CD20 is an important cell surface receptor that is used for target therapy of B cell lymphoma and some related blood diseases due to vital function of CD20. In previous studies, a Rituximab based humanized single chain variable fragment [scFv] antibody showed good reactivity against B cell related cancer cells. But this recombinant protein produced Inclusion Bodies [IBs] in Escherichia coli [E. coli] cytoplasm .The aim of this study was to investigate the effect of coexpression with cytoplasmic chaperones on expression and solubility of humanized anti-CD20 scFv in E. coli