In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants

ABSTRACT

A protocol is established for regeneration of the economically important cut flower plant, Gypsophila paniculata L. using shoot tips explants. Multiple shoots were obtained on MS-medium fortified with 0.5 mg/l each of NAA and BA. Addition of 10 g/l agar promoted shoot proliferation and reduced the degree of shoot vitrification. Transfer to 3 mg/l IBA containing medium produced optimum root initiation and development. The produced plants as well as intact plants were subjected to the random amplified polymorphic DNA [RAPD] analysis. Using 9 primers, the total number of amplification products generated by PCR amplification was 142 bands [15.7 bands per primer], of which 7.74% showed polymorphism. The analysis of bands recorded, showed 92.25% similarity. The results indicated that variation at time DNA level has occurred during in vitro culture of Gypsophila but in a very low level