Optimization of cultural conditions of penicillium griseofuhvum for the production of penicillin griseofulvin

ABSTRACT

The present study is mainly concerned with the production of Penicillin griseofulvum by Penicillium griseofulvum NRRL 2300 in Shake Flask Technique. The production of antibiatic was carried out in cotton wool plugged 250 ml flasks. 24 hours old, vegetative inoculum was developed in conical. flasks, containing 100 ml of basal medium. Fermentation, for antibiotic synthesis was carried out for 312 hours at 2S degree C. Effects of different nitrogen and carbon sources for the formation of antibiotic were evaluated. Maximum production of antibiotic was observed in the presence of corn steep liquor, which was used as a source of nitrogen. Maximum production of penicillin griseofulvin was also observed in the presence of sucrose as a source of carbon; whereas it was decreased in the presence of other carbon sources like lactose and glucose. Other conditions such as temperature and pH were also optimized for the production of griseofulvin