Immunohistochemical detection of fibronectin in bone wound healing of ovariectomized guinea pigs receiving raloxifene

ABSTRACT

As Fibronectin [FN] performs essential roles in bone Wound healing and as osteoporosis is considered a complication of ovariectomy, this study was conducted selecting the FN as an immunohistochemical marker in bone wound healing in ovariectomized Guinea Pigs [GP] versus another ovariectomized GP receiving raloxifene as estrogen replacement therapy. Twenty four adult females Guinea Pigs were used, they were divided into two equal groups, control and experimental. Both groups were subjected to ovariectomy. The animals in each group were subjected to the 2[nd] operation to prepare bilateral standardized bony cavity adjacent to the midline in the submental area of the mandible, of each. After the second operation each animal in the experimental group was received 2mg daily dose EVISTA, [Raloxifene hydrochloride]. Four animals from each group were sacrificed at 15, 30 and 45 days from the second operation, bone specimens were taken for processing and immunohistochemical staining with antimouse cellular FN monoclonal antibody. The results of this study had revealed FN detection in the experimental group bone sections at 15, 30 and 45 days in the bony trabeculae of the healed cavity and in the marrow spaces but in the control group; it was absent in the bony trabeculae at the same intervals. Thus we can conclude that; raloxifene is a new potent medication as estrogen replacement therapy in bone wound healing after ovariectomy