Molecular detection of blaVIM Metallo-beta-lactamase producing clinically isolated Pseudomonas aeruginosa from tertiary care hospital, Faisalabad

ABSTRACT

Metallo-beta-lactamases [MBLs] producing Pseudomonas aeruginosa are major threat for public health. They produce resistance against various antibiotics and remain low or no therapeutic options. A total of 200 clinical isolates of P. aeruginosa were collected from tertiary care hospital, Faisalabad. Isolates were sub-cultured on basic and selective media and confirmed by API 20NE. Phenotypic detection of carbapenamase, MBLs, antibiogram and MIC were determined as per CLSI guidelines. Molecular detection of blaVIM was performed using specific primers by PCR. Among 200 P. aeruginosa, majority [n=82] were isolated from pus samples followed by 28 from tracheal aspirates and 27 from sputum. Out of 110 [55%] MDR P. aeruginosa, 12 [11%] were positive for MHT and MBLs and blaVIM was identified in MBL positive isolates. Antibiogram revealed that all the isolates were resistant to beta-lactam drugs including carbapenems followed by 95% to levofloxacin, 67% to doxycycline and more effective drugs were tigecycline and colistin. MIC value for imipenem drug was 16micro g/mL and 8micro g/mL against 6 and 5 isolates respectively while MIC value for meropenem against 6 and 3 isolates were 8micro g/mL and 16micro g/mL respectively. Our study concluded the high prevalence of blaVIM producing P. aeruginosa in our clinical settings