[Synergistic effects of cyclosporin a and hematopoietic growth factor on human bone marrow cell multiplication in longterm bone marrow culture and colonal culture in semi-solid ayar medium]

ABSTRACT

Introduction: Cyclosporin A [CYA] is a potent immunosuppressive drug widely used in transplant settings due to its specific inhibition of T-cell activation. Several Studies have reported the synergistic effect of CyA with hematopoietic growth factor in colony culture of mice bone marrow. We Studied the effect of IL-3, IL-6, SCF, CyA and nutrition medium [ condition medium - PHA] on the development of hematopoietic cells of human bone marrow because this unique cyclic endecapeptide has been successfully utilized in organ transplantation for graft rejection and graft versus host disease and also since CYA stimulates colony formation by hematopoietic cells in vitro