[Evaluation of the relationship between ovarian dysgermlnomas and the results of DNA analysis]

ABSTRACT

This paper studies the relationship of DNA analysis ploidy and stage of ovarian dysgerminoma and its prognosis with flowcytometric evaluation. The files and specimens from 24 cases [mean age 18/2+/-4/5 years] seen at Mirza Kouchak khan and Imam Khomeini hospitals between 1992 and 1997 were assessed and the patients followed for period of five years. There were 11 cases [45/8%] in stage I, 2 [8.3%] in stage II, 7 [29.2%] in stage III and 4 [16.7%] in stage IV. DNA aneuploidy was revealed in 13[54.2%] of tumors. There were 6 [15%] deaths, 7 [29.2%] recurrences and 11 [45.8%] free from the disease during the follow-up. DNA ploidy was significantly related to prognosis [p = 0.0006] and stage [x[2] = 15.9, p = 0.04]. The deaths and recurrences in cases with DNA aneuploidy were 30.5% and 53.8% respectively; only 7.7% were free. The most frequent aneuploidy was in stage III and IV [85/7% and 75% respectively]. Within microscopic data, mitosis, pleomorphism, necrosis and hemorrhage significantly related to DNA ploidy [p< 0.05]. The frequency of aneuploidy was greater in mitosis > 8 in HPF, higher pleomorphism and presence of necrosis and hemorrage. Our study revealed that DNA aneuploidy in ovarian dysgerminoma is related to higher staging and poor prognosis