Evaluation of using potassium pyroantimonate in retention of 45Ca in enamel and dentine forming cells

ABSTRACT

To evaluate the effect of potassium pyroantimonate [PPA] on the retention and localization of [45]Ca in both ameloblasts and odontoblasts, six frogs were injected with 5uci/gm body weight of [45]CaCl[2]. Pairs of the animals were sacrificed 5 min, 1 h and 24 hs post injection. Blocks of the developing teeth were dissected and fixed in 4% glutaraldehyete with and without PPA [control] and processed for autoradiographic examination. Sections of 36 teeth in the same stage of development were selected and the means of net silver grain counts /Um[2] of PPA treated and control tissues of similar time interval were compared for statistical significance. Counts in PPA specimens were consistently higher for both types of cells and in all time intervals with statistical significance in differences between experimental and control mean counts. These findings suggest the formation of PPA complexes with calcium allowing its retention without interfering with its distribution either in ameloblasts or odontoblasts