Sensitive colorimetric method for the determination of thymol and eugenol in volatile oils

ABSTRACT

A simple, rapid and direct method for the determination of thymol and eugenol in volatile oils was presented. It was based on the reaction of these compounds with 3-methyl-2-benzothiazolone hydrazone hydrochloride in the presence of cerric ammonium sulfate and direct measurement at 510 nm. The results obtained were encouraging and reasonably reproducible with a relative st and ard deviation less than 1%