Effect of horse dung extract on the growth and some metabolic changes of chlorella vulgaris

ABSTRACT

Chlorella Vulgaris was cultured under controlled laboratory conditions on different concentrations of horse drug extract prepared either by soaking, boiling or autoclaving. The number of cells, protein chlorophyll contents and dry weight were determined as growth parameters. Results revealed that 0.2% waste extract obtained by soaking was the best one for algal growth. Almost all waste concentrations prepared by any of the previous treatments supported higher growth compared to that with Bold's basal medium [B.B.M.] conducted as a control. Chlorella cells absorbed considerable amounts of organic-N compounds, nonreducing, and lesser amounts of both reducing sugars and nitrate from the waste medium, while about 41% of the initial nitrate content were absorbed from the control medium. The transfer of Chlorella cells to the waste medium resulted in an elaborate increase in all the intra cellular nitrogen fractions tested Ammonia was detected only in cells grown on the waste medium and carbohydrate content decreased by about 32% of its initial value