Production of alpha-amylase from bacillus lentus

ABSTRACT

A number of nutritional factors affecting growth and alpha-amylase by amylase by Bacillus lentus were investigated. The production of enzyme increased with increase of temperature reaching its maximum between 40°C - 45°C after 60h incubation when cultivated in a medium with 30 g/1 starch as carbon source. Supplementation of the medium with different carbon sources did not enhance either enzyme activity or growth. Of the nitrogen sources examined a mixture of peptone, yeast and beef extracts stimulated growth and yielded the highest enzyme production