Determination of chloramphenicol and thiamphenicol in the presence of their degradation products using first derivative spectrophotometry

ABSTRACT

A method is presented for the determination of chloramphenicol and thiamphenicol in the presence of their degradation products using first derivative spectrophotometry by measuring the absolute value of the first derivative curves at the zero contribution of the corresponding degradation products, the concentration of the intact drug can be calculated directly without interference of the degradation product. The method was proved using laboratory prepared mixtures of the intact drugs with their degradation products