Investigation of enzymes, enzyme inhibitors and glycoprotein as biomarkers for discrimination between stored and fresh onion seeds by electrophoresis

ABSTRACT

Discrimination between cultivars as well as between stored and fresh onion seeds were corned out by detection of enzymes [Peroxidase and superoxide dismutase] enzyme inhibitor [Trypsin inhibitor] and glycoprotein after electrophoresis The glycoprotein bands of 30 67 and 80 K Da in Tris-borate buffer and the band of 26 K Do in water extract were used as a marker for stored onion seeds. The Trypsin inhibitor band of 30 K Da still used as a marker for stored onion seeds. The 50 K Da band could be used as trypsin inhibitor marker for cultivars identification using gelatin as substratein the investigation proved that glycoprotein and trypsin inhibitor protein bands could used as biomarker for differentiation between onion seed cultivars. Monitoring the activities of peroxidase and superoxide dismutase enzymes in the water or buffer extracts of onion seeds were considered as a good tool for the discrimination between cultivars