Human pregnancy following sperm antibody separation technique and sperm intra-uterine transfer

ABSTRACT

Microscopic examination of the seminal fluid of infertile patients who gave positive kibrick and/or F-D tests also revealed massive sperm clumping and agglutination. Positive correlation was observed between infertility and sperm antibodies. Sperm antibodies were also found in the cervical mucus of infertile females. Sperm clumping was treated by dilution with 50% fetal calf serum followed immediately. by physical and mechanical separation of sperm clumping several times. The sperm motility was increased from 10% to 50%. Ovarian stimulation was induced by clomipgene citrate [100 mg from day two to day six of the cycle] and human menopausal gonadotropin [two ampoules from day 7 to 11 of the cycle] followed by 5000 to 10000 International Units [IU] of Human chorionic gonadotropin when the diameter of the ovarian follicles was more than 1.8cm and the concentration of estradiole was more than 300pg/ml. Following the separation of sperm clumping, the hyperactive sperm cells were selected through a simple layer technique. A concentration of one to two millions of sperm cells was removed from the top and middle rich sperm fraction and transferred in less than 200 micro liter of sperm culture medium into the uterus by special catheter. A total of ten transfers was done and five pregnancies induced. Out of five pregnancies, three were aborted within the first trimester and two pregnancies gave birth. The first baby was a male born on 31 of January and the second case was a female born on first of February 1990. Both babies are healthy and weighing more than 3000 grams and the lengths are also more than 49cm. It was concluded from the results of this study that the technique used under the experimental conditions of the present study was found to have a significant value in the treatment of infertile couples