Electrophoretic separation of two aedes caspius pallas forms from Egypt

ABSTRACT

Isozyme banding patterns of the recently recognized autogenous stenogamous and anautogenous eurygamous sexually isolated Aedes caspius forms were investigated using horizontal starch gel electrophoresis of 5 enzymes. The esterase assay clearly separated the 2 forms, a faster band being characteristic of autogenous individuals. Other tested enzymes were encoded by 5 loci that were highly polymorphic. The "malic" enzyme locus was unambiguously diagnostic, specific, non-overlapping alleles at that locus were revealed in each form. Other enzyme loci screened exhibited at least 2 alleles common to both forms, allelic frequency differences at these loci could not separate the 2 forms except at the cathodal MDH-1 locus [malate dehydrogenase assay]. Probability of correct diagnosis at the MDH-1 locus was as high as 93% so that it may be used as a confirmatory test