Chromosomal proteins in pulmonary alveolar macrophages

ABSTRACT

In this study the nature of chromosomal proteins, histones and nonhistone in resident alveolar macrophages was investigated in comparison to peritoneal neutrophils and calf thymus proteins. Cells were obtained by lavaging the lung and after purity determination they were subjected to fractional extraction procedures. Proteins were then analyzed on SDS polyacrylamide gels and densitometric scans were obtained. The results show that in macrophages, the pattern of histone and nonhistone proteins were similar to thymus proteins, two distinct and specific proteins Hi[°] and HMG14b and also a protein with a molecular weight of l2kd [Hx] were present. These data suggest a different chromatin protein pattern in pulmonary alveolar macrophages