Immuno fluorescence study of renal lesions in diabetes mellitus

ABSTRACT

Percutaneous renal biopsies were performed in 30 diabetic patients, who presented with proteinuria. The tissues obtained were studied for evidence of an immune lesion using immunofluorescence techniques. We could not demonstrate any Consistent pattern of binding of FITC labelled polyvalent, monospecific IgG, IgA, 1gM and labelled bovine insulin antisera in various diabetic renal lesions. The binding of labelled insulin could be observed even in biopsies obtained from patients, who had never received exogenous insulin therapy. we, therefore, conclude that there is no evidence to support an immune pathogenesis in the morphogenesis of the diabetic renal lesions, nor do these lesions occur as a result of insulin therapy