Study of some metalloenzymes and its related trace elements affecting lipid peroxidation in chronic renal failure

ABSTRACT

The level of lipid peroxidation is affected and enhanced in chronic renal failure [CRF]. This could be estimated by measurement of malonlydialdehyde [MDA]. This increase in lipid peroxidation could be either increase in free radicals or due to defective antioxidants, e.g. glutathione peroxidase [GSH-Px] and superoxide dismutase [SOD] and its related trace elements. This study was carried out to detect the lipid peroxidation level and the defect in the activity of antioxidants, if present, in chronic renal failure patients both on conservative medical treatment and those on maintenance hemodialysis. Of the present study were divided into 3 group [I] CRF on maintenance hemodialysis, [II] CRF on conservative medical treatment, and [III] control group. Each group was of 10 patients. The study showed significant increase in serum copper in group I as compared to groups II and III, a significant decrease in serum zinc and selenium in groups I and II when compared to group III and a significant increase in serum zinc and selenium in group I when compared to group II. Erythrocyte MDA was found to be clinically higher in group II when compared to I and III and in group I when compared to III. Erythrocyte SOD was significantly decreased in groups I and II as compared to III. Erythrocyte glutathione peroxidase was normal in group I and II. These results confirm incomplete disturbance in the enzyme system incorporated in free radicals scavenger in CRF patients. It is concluded that dialysis is harmless to the dialyzed patients and imposes no oxidative stress on them