Lipids-lipoproteins-apolipoproteins prediction of coronary artery disease

ABSTRACT

Epidemiological studies have established that plasma levels of certain lipids and lipoproteins are risk factors for atherosclerotic cardiovascular disease. The methods used to study lipoprotein metabolic disorder were based on physicochemical criteria [electrophoresis, ultracentrifugation, polyanionic precipitation]. But the recent demonstration of the preponderant role of apolopoproteins in lipoprotein metabolism makes it essential to develop a method of analysis lipoproteins at the molecular level. Using immunoenzymometric methods and well characterized monoclonal antibodies directed against Apo B,we observed a significant increase in all Apo B particles but particles screened by two antibodies [BL3] and 6 B] contribute to a better discrimination between patients and controls. During a double blind randomized placebo-controlled study, we observed that lipanthyl ' induced different effects on the different subpopulations of lipoproteins. The most striking difference was the decline in particules recognized by BL 3. lmmunological methods seem to be the most appropriate and should allow us to study lipoprotein metabolism more closely