Quantitation of floctafenine by high pressure liquid chromatography [HPLC] and spectrophotometric methods

Two methods for the quantitative determination of floctafenine are described. The first, a spectrophotometric procedure, is based on the formation of a red Schiff-base between floctafenine and p- dimethylaminocinnamaldehyde [p-DAC] after reduction with zinc powder and hydrochloric acid in ethanol acidic medium at room temperature. At the maximum absorption of 541 nm, Beer-Lambert's Law was adhered to over the 1-9 mug/ml range [epsilon max 3.99 x 10 4 l/mol/cm]. The second method involved the application of HPLC, C-18 column, with a mobile phase consisting of 82.5: 17.5 methanol: water at a flow rate of 1 ml/min. Samples dissolved in methanol were estimated by the measurement of peak height using UV detection at 254 nm. The results obtained with the two proposed methods revealed their successful application for the quantitative estimation of floctafenine in bulk drug and as tablets with good accuracy and precision