Pattern of amino acids and proteins of blood serum in vaccinated chickens with gumboro disease vaccine

ABSTRACT

Twenty blood samples were taken from 10 vaccinated chickens with Gumboro disease virus [vaccine strain] and from other 10 non- vaccinated [control] chickens, serum was separated for determination of free amino acids, total protein and protein fractions. Recorded results concerning free amino acids revealed that there was a very highly significant decrease in tyrosine level, high significant decrease in the levels of proline, cysteine, valine and phenylalanine, significant decrease of lysine concentration. Whereas, the level of glutamic acid showed a highly significant increase in samples collected one day post vaccination with Gumboro vaccine. The amino acids [threonine, serine and valine] showed very high significant decreased levels. Both methionine and lysine were significantly decreased. While, glutamic acid showed significant increased value. Serum proteins showed a significant decrease in total protein level which associated also with a very high significant decrease of albumin fraction. In contrast, alpha globulin fraction revealed a very high significant increase, the A/G ratio was decreased in samples collected one day post vaccination with Gumboro vaccine. Ten days post vaccination, total protein showed a high significant decrease in accordance with the very high significant decrease of albumin fraction. But both alpha and gamma globulin fractions showed a very high significant increase, compared with their control values. The A/G ratio was decreased