Recovery and transfer of Egyptian buffalo embryos using non-surgical technique

ABSTRACT

The present study was undertaken to apply and evaluate the new technology of embryo transfer system and its success in Egyptian buffaloes. Eleven buffalo-cows were used in this study and were assigned into two groups. The first [n=8] was for donors and the second [n=3] was for the recipients. The animals were superovulated and synchronized for estrus using a total dosage of 50 mg FSH-p [Shering Corporation, USA] and 25 mg Lutalyse for each animals respectively. The animals were naturally mated at least 2 times 36-80 hours [hrs] after Lutalyse injection. The embryos were collected and evaluated at day 5 to 5.5 after estrus. The good transferable embryos were transferred non-surgically to the uterine horn ipsilateral to the corpus luteum [CL] to 2 recipient buffaloes. The percentage of animals that responded to superovulation was 100%. Eight embryos were collected [transferable] and the average number of transferable embryos/ animals was 1.6. One animal was confirmed pregnant 45 days after estrus. The present results revealed that the technique of embryo transfer in Egyptian buffaloes needs more investigations; at the same time the present protocol was found to be satisfactory to reduce the problems facing the embryo transfer technique in Egyptian buffaloes