Rapid carbohydrate utilization test for haemophilus influenzae

ABSTRACT

The carbohydrate fermentation pattern of 150 Haemophilus influenzae strains was observed using the rapid carbohydrate utilization technique [RCUT], devised originally for differentiation of Neisseria species. Twenty-five sugars were tested. The RCUT proved to be as sensitive and reproducible as conventional method, when the results of fermentation of 7 of the currently used sugars in differentiating Haemophilus species were compared. Moreover, the RCUT carried the advantages of being rapid and independent of growth, and the medium was clear giving profound color changes. The reagents were compact, stable and readily maintained for months