Simultaneous determination of papaverine hydrochloride/anlagen mixture in presence of homatropine methyl bromide in pharmaceutical preparations

ABSTRACT

In this work, a simple first-derivative [D1] method for the simultaneous analysis of papaverine hydrochloride/analgin [dipyrone] mixture in the presence of homatropine methyl bromide, was described. D1 spectrum of papaverine hydrochloride exhibits at 257 nm a definite peak where analgin and homatropine methyl bromide reads a zero [D1] value. Similarly, analgin exhibits a definite peak at 287 nm without interference from papaverine hydrochloride and homatropine methyl bromide. The method was applied successfully to the analysis of supergine ampoules. The mean percentage recoveries +/- SD were found to be 100.66 +/- 0.43, 100.64 +/- 0.87 for papaverine hydrochloride and analgin, respectively. Analgin was determined iodimetrically followed by spectrophotometric measurement of papaverine hydrochloride in the solution. Oxidation of analgin resulted in a blue shift in its spectrum, this permits direct spectrophotometric determination of papaverine hydrochloride without any interference. The method was applied to the analysis of supergine ampoules. The mean percentage recoveries +/- SD were found to be 100.10 +/- 0.67 and 99.49 +/- 1.71 for papaverine hydrochloride and analgin, respectively. On applying both of the proposed methods the results obtained were verified by the standard addition technique, where good percentage mean recoveries with relatively low deviations were obtained