value of detection of HBVDNA in hepatocellular carcinoma patients using PCR technique

ABSTRACT

Thirty patients with primary HCC and 10 healthy controls were subjected to the study. Serological markers and HBV DNA were detected. In the patient group, HBsAg was positive in 21/30 [70 perecent] of cases,while anti-HBc was positive in 19/30 [63 percent] of cases. Both markers were positive in 17/30 [56,7 percent] of cases while both markers were negative in 7 of the cases. Only one patient of the control group was positive for HBsAg. Using the PCR technique to detect HBV. DNA in PBMC 27/30 [90 percent] of the patients group had detectable amounts, while none of the control group had HBV, DNA. This showed the close association between chronic HBV infection and disintegration of viral DNA which causes disarrangement of host DNA and produce malignant changes. The aim of this present work is to confirm the correlation between HBV and Hcc by detecting HBV-DNA by the polymerase chain reaction technique [PCR]