Binding of the antitumor drug adriamycin to DNA-histone complexes

ABSTRACT

Isotherms of the binding of the anthracycline antibiotic, adriamycin [adriblastin], to DNA histone complexes was studied by means of spectroscopic analysis. The results indicated that [a] binding of adriamycin to histones reduced the interaction of histones with DNA, [b] binding of the drug to DNA did not change the binding affinity of histone to DNA and, [c] in the explored binding range of r<0.1 the binding of adriamycin to DNA-histone complex proved to be anticooperative with n values of 0.32 for the interaction of histone with DNA-drug and 0.26 for the binding of DNA to histone-drug complex. The results suggest the possible participation of histones in the DNA-drug complex formation