Biophysical studies of hemoglobin absorption spectra in subjects from hormonal disturbance

ABSTRACT

Absorption spectra of oxy hemoglobin [1.3 X10 [5] M] of 116 females receiving oral contraceptives together with 20 controls of parallel age and in the same socioeconomic conditions were studied. Band of spin slate at lampda .max =578 nm., or heme-heme interaction is greatly decreased in the case of contraceptive treatment as well as in Juvenile diabetes. This finding indicates a change in oxygen earring capacity of hemoglobin. Decreases in Sorest band as well as at lampda max. 542 nm. and 578 nm are attributed to the changes in Fe-N bond lengths. No shift was detected in the visible region of alpha the hemoglobin absorption spectrum due to aggressive treatment with contraceptives and absolute deficiency of insulin. A slight shift which was only observed in the free from spectrum of subjects taken contraceptives proved that oxyhemoglobin exist in intermediate spin state. Stabilization of hemoglobin or hemoglobin interaction was greatly affected only in subject with juvenile diabetes, existing in pre-coma. Low equilibrium constant of spin state of these subjects was noticed concomitant with high free energy. Attention should be drawn to decrease in the ratio of lampda 578/lampda 542 and the appearance of a new band at 630 nm which indicates the conversion to methemoglobin suggesting an effect in oxidation-reduction potential, namely methemoglobin reductase system