Studies on the development of new castle disease killed vaccine

ABSTRACT

Effect of beta propiolactone [BPL] as inactivating material on different strains of Newcastle disease virus [NDV] [velogenic, mesogenic, lentogenic, La Sota and B1] was investigated. Oil adjuvant inactivated vaccine inactivated with BPL was prepared using La Sota strain of NDV. The end concentration of BPL vaccine was 0.2%. The prepared vaccine proved to be sterile and safe as controlled, respectively, by bacteriological test and inoculation into embryonated chicken eggs and 5-week old chickens. The immune response HA titer of chickens inoculated s.c. with BPL inactivated oil adjuvant NSD vaccine at 7-week old, gradually increased for the 1st week, reached maximum at the 4th week after inoculation [GM 7.1], then gradually dropped to 6.4 at the 6th week post vaccination [PV]. Challenge test at 3 weeks and 6 weeks PV resulted in 100% protection in vaccinated chicks challenged 3 weeks PV and 90% protection in vaccinated chicks challenged 6 weeks PV